Differential Stains For Histological Analysis (Enamel,Dentin,Cementum) Of Teeth And Bone Using Van Gieson And Masson’s Trichrome Stain.

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Padmapriya, Abilasha R, Lakshmi.T.A

Abstract

Aim:To analyse the histology of teeth(enamel,dentin,cementum) and bone using van gieson and masson’s trichrome stain.


Introduction: Identification of hard tissues is a challenging task,various methods have been done and demonstrated. Identification of calcified mass and pathological lesions is very difficult. Staining is an additional technique which shows contrasting images under a microscope. It provides the histological features of pathological tissues.


Materials and methods: 5 teeth samples were taken from dental clinics. Prepared slides of sections of teeth using van gieson and masson trichrome to identify siaolith ,osteomyelitis and odontome and observed under electron microscope.


Result: Tissues stained with Van gieson showed collagen-blue, muscle and cornified epithelium-yellow, nuclei-blue-black. Tissue stained with masson trichrome showed


collagen-blue, nuclei-black, muscle,cytoplasm and keratin-red.H&E stained tissue showed Nucleus-purple hematoxylin, cytoplasm-pink eosin.


Conclusion :  H and E and Masson's trichrome stain are cost-effective. It identifies hard tissue components such as bone, cementum, and dystrophic deposits from stromal components by staining them separately.

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