Molecular Profiling And Antioxidant Potential Of Citrus Limon (L.) Burm.F Fruits

Abstract

Citrus limonL. Burm F. was subjected to preliminary screening for phytochemicals, followed by molecular characterization using GC-MS and antioxidant analysis,using aqueous, petroleum ether, ethyl acetate, chloroform and ethanol extract of fruits. Qualitative analysis showed the presence of phenols, flavonoids and triterpenoids was present in ethanol, ethylacetate and aqueous extract with maximumtotal phenolic content of of 40.1 ± 0.7 mg/100g, flavonoid content analysis result showed 65.8 ± 0.8 mg /100g, and total terpenoidof 44.7 ± 0.16 mg/100g in the ethanol extract of fruits. The antioxidant potential of ethanolic fruit extract evaluated through DPPH assay exhibited an IC 50 value of 85.83 % strong antioxidant activity when compared to other solvents. While SARS assay of the ethanol extract had IC 50 values of 84.38 %. The chloroform fruit extract of plant showed very low antioxidant activity. The GC-MS analysis ofethanolic fruit extract revealed the presence of 9 compounds which were eluted at various interval of time. The chemical compounds Urs-12-en-3-ol, acetate (3-beta) showed the highest sharp peak of 57.97 % at a retention time (RT) of 19.759 minutes, indicating the presence of the compound Urs-12-en-3-ol, acetate(3.beta). The smallest peak with the retention time (Rt) of 13.178 minute had the corresponding compound identified as 9-Undecenal, 2,10 –dimethyl.