The phylogenetic groupings of Escherichia coli isolated from human and farm animal feces in Basrah district, Iraq

Abstract

 The current work objective is to detect and differentiate Escherichia coli isolates from the feces of livestock animals and humans. Between September 2018 and January 2019, a total of two hundred and sixty-four fecal swabs and samples were gathered from different regions of Basrah. Eighty-five cow samples, ninety-four human samples, and eighty-five sheep samples were among the samples. The existence of E. coli was determined using conventional microbiological testing and molecular approaches (by PCR for amplification of the uidA gene). The findings of these procedures revealed that 50 (18.9%) of the examined samples had E. coli.

 The E. coli isolates were then tested for phylogenetic groupings using quadruplex PCR employing four genes (chuA, yjaA, TspE4.C2, and arpA). In this investigation, seven phylogenetic groups were discovered, with group D having the largest proportion (44%) and group E having the lowest (20%). Other isolates belonged to groups F, A, B1, and C, with 8 percent, 6 percent, 4 percent, and 2 percent, respectively. However , 16% of isolates were unfit for other types (Unknown ).