The Detection And Genotyping Of The Human Papillomavirus In Vietnam

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Sang Tien Trieu , Khoa Van Tran , Trang Thi Nguyen , Giang Truong Ngo , Tung Thanh Nguyen , Hoang Minh Ngan , Tien Anh Ngo , Vu Huong Ly , Vu Van Tam

Abstract

Background: Human papilloma viruses (HPVs) are the major etiological agents of cervical cancer which is a common woman’s cancer type. More than 100 HPV types have been identified; approximately 70% are capable of infecting genital mucosa. A.


 


Materials and Methods: In the presented study we have used the Papanicolaou smear (PAP test) is used to detect epithelial abnormalities. More other the PCR-test assay combined with Reverse Dot Blot Hybridization has allowed for accurate detection of HPV and specific types of infection. Have been tested 1403 patient samples at Vietnam Military Medical University (Hanoi, Vietnam) to screen and identify HPV genotypes.


 


Results: Was detected, the 169 samples (12.03%) were positive and 152 samples (11.19%) had unidentified results of the PCR-test was noted positive samples. And the most frequently found three HPV subtypes were 18 (26.63%), 16 (17.58%), and 11 (13.59%). This group was followed by HPV subtypes 6 (9.78%), 81 (8.15%), 45 (5.43%) and 82 (3.26%). Other genotypes were detected at frequencies more over 4% and 6 genotypes occur only once.


 


Conclusion: The results have indicated the prevalence and diversity of HPV infection in specimens. It is extremely important is PCR-test and Reverse Dot Blot Hybridization using in the HPV diagnosis and strain determination.

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