Protease Production And Purification In Bacillus Cohnii Isolated From Vegetable Market Waste

Abstract

Using skim milk agar plates, proteolytic bacteria were identified from vegetable waste dumping soil. Bergey's handbook was used to select and define the isolate with the highest activity. It showed the most resemblance to Bacillus cohnii in 16S rDNA study, and the sequence was deposited in Genbank. The strain SSK1 was cultivated on a modified production medium with sludge as a substrate to produce protease. For maximum enzyme synthesis, many culture factors were tuned. With 1% inoculums, peak proteolytic activity was reported at pH-8.5 and temperature of 50°C. Tween-80, SDS, and Tween-20 had the strongest inhibitory action, followed by Cetrimide. Q-Sepharose was used to homogenise the protease, revealing a molecular weight of 35 kDa. At pH-9 and 55°C, the enzyme was more active and stable.