Analysis the antibacterial activity Pseudomonas aeruginosa pigment on Escherichia coli

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Dr.V.Jeyanthi Kumari

Abstract

Biodegradability, non-toxic nature and therapeutic nature of the natural pigments, colorants and dyes make an alternate and attractive source for human use. The current study aimed to screen and identify pigment producing Pseudomonas aeruginosa from garden soil using pseudomonas isolation agar and its species level was identified under the guidance of Bergey’s manual. Different incubation times were provided to optimize its maximum pigment production. There was a slight colour change in the media observed, for the first three days during the incubation of P.aeruginosa in nutrient broth. The colour change was notable from the fifth day onwards. On the seventh day, it was dark green and continued on until the end of the incubation time. The validity of this experiment was demonstrated by colorimetric method. The pigment concentration was low (0.076±0.18) during the third day of colorimetric reading. The concentration of pigments was gradually increased at the time of the fifth and seventh day of colorimetric observation. The concentration of pigment production in optical density was increased at 9th day observation (1.873±0.16). The colorimetric reading in the following days was more or less the same. The density of the pigments remained intact.

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