The Immunomodulation Effect Of Purified Resveratrol Extracted From Black Grape Skin Cultivated In Iraq

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Zainab Yaseen Mohammed Hasan, Mohammad Mahmoud Farhan AlHalbosiy, Raghad K. Al-Lihaibi

Abstract

 


The Iraqi black grape is rich with active ingredient that regarded a promise phytochemical indicated to treat and protect many disease and human disordered as well as acted as immune boosting ; this natural substance called (Resveratrol). The study employed extraction, purification, analysis for insuring its purity, and evaluate the purified resveratrol activity on normal human lymphocytes culture as an immune boosting by affecting the interleukins level causing immune modulation effects.


 


Results showed that the black grape skin contain 7mg%W/W resveratrol extracted as two isomers(cis & trans)as pure crystals.  The resulting crystals purity was insured in comparison with standard resveratrol SigmaRby  ( HPLC),  (CHO) checking analysis , and the functional groups by infrared FTIR test, all tests as standard resveratrol assay values. The immune  modulation of this purified natural substance was obvious through decreasinglevel of IL 2 & IL 12 significantly within  2&4 hours, while the expression of IL10 started after 4 hours exposure with and dcreasing in IL-6 level at low concentrations of resveratrol significantly


 


Conclusion: black grape skin cultivated in Iraq is rich with resveratrol that has the ability to regulate and strengthen with boosting the immune system, to stand against diseases and fight abnormal cells in the body, as it works as an immunomodulation agent.

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