Identification Of Some Olive (Olea Europaea L.) Cultivars Cultivated In North Of Iraq By Using (RAPD) Markers

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Muna O. M. Shehab, Aamer M. Al-Ma'thidy, Talal Taha Ali

Abstract

In this study the molecular Identification of (6) cultivars Olive (Olea europaea L.) namely “Cormal”, Santa Catrina”, Khoderiy”, “Ducal”, “Sorani”, Baashiky” used Random Amplified polymorphic DNA (RAPD) markers for identifying the genotype relation among cultivars were analyzed with (10) RAPD which generated (360) random bands. Some of them were variant, and other were distinct. Fragment size ranged from 170- 1737 bp.


The data were enteredinto the computer, and the privates statistical application NTSYS- PC
wasutilized for this sort of study,which revealed that the cultivars genetic distance was in the
range of (0.191-0.406).The distancebetween “Baashiky” and “Sorani”cultivars has been the greatest (0.406),whereas the distance between “Khoderiy” and “Santa Catrina”cultivarswas the smallest(0.191). UPGMA (i.e. Unweighted pair Group Method Analysis) yielded a dendrogram and similarity matrix, which revealed the cultivars had been separated into sub-major and major groups clustares based on the genetic distance value.

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