Molecular Detection Of Bifidobacterium Dentium From Patients With Tooth Caries

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Fatima Malik Al-Rubea, Reyam Abdul Khuder Mohammed, Mohammed M. Barrak, Ahmed Jaber Aboob

Abstract

The goal of this study for molecular detection of Bifidobacterium dentium from patients with tooth caries.Samples were taken from the root caries lesions of nine distinct patients, which yielded 150 samples. fastidious anaerobe broth was used to dilute the samples.Then identifications of bacteria were done by using biochemical tests. DNA of suspected bacteria were extracted and two primers were used for detection of B. dentium (CI and 16S rDNA). According to conventional techniques and biochemical analyses, 28 isolates were B. dentium. whereas molecular identification identified 24 (85.7 percent) of the isolates as being of B. dentium. To find particular genes, all the isolates in this investigation were subjected to PCR using species-specific primers and a PCR method called conventional PCR.


In conclusion, B. dentium was detected in a high percentage in dental caries patients which were diagnosed by bacteriological and molecular methods.

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