In Vitro Study Of Anti-Diabetic Effect Of Broccoli Mediated Selenium Nanoparticles

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Sudarsan R , S. Rajeshkumar , Dhanraj Ganapathy , Revathi Duraisamy , Ashok. Velayudhan

Abstract

The SeNPs have paved their way into the field of biomedicine by serving as an anticarcinogenic agent, antidiabetic agents, antioxidant agent, or as a nutritional supplement, also as a drug supplement against AIDS. Broccoli accumulates Se many-fold beyond the concentration of Se in the soil. The present investigation aims to evaluate the possible antidiabetic effect of selenium nanoparticles mediated by broccoli. Preparation of nanoparticles powder was done by centrifuging the NP's solution at 8000 rpm for 10 minutes, then collected, washed twice with distilled, water dried at 600 C and stored. SeNP concentrations of 10,20,30,40 and 50 μL were preincubated with 100 μL of alpha-amylase solution (1 U/mL) at room temperature for 30 min. About 100 μL of starch solution (1% w/v) was then added to it and the mixture was incubated at room temperature for 10 min. About 100 μL of 96 mM 3,5-dinitrosalicylic acid reagent was added to it to stop the reaction and the solution was heated in a water bath. Control was maintained where equal quantities of the enzyme and extract were replaced by a sodium phosphate buffer maintained at a pH of 6.9. The reading was measured at 540 nm. The experiment was performed thrice. Acarbose was used as a positive control and the percentage of inhibition was calculated. The current study demonstrated results in which Se-

NPs had an antidiabetic effect close to that of the standard used. 57.2% of alpha amylase enzyme inhibition was seen at 20μl concentration which was significantly comparable to the standard acarbose used. From the current study we can confer that Selenium nanoparticles has an inhibitory effect on alpha amylase and this inadvertently has a therapeutic effect on Diabetes Mellitus by controlling the blood glucose level. SeNPs synthesized using broccoli have an evident antidiabetic potential. They show a considerably good amount of alpha-amylase inhibition with respect to acarbose. Thus, SeNPs could provide a possible alternative for conventional antidiabetic drugs in the future.

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