Isolation And Molecular Detection Of Klebsiella Pneumoniae Isolated From Patients With Oral Inflammation Plus Pneumonia And Its Effect On Secretory Iga Levels

Abstract

The study's purpose was to find out whether Klebsiella pneumoniae could be isolated and detected molecularly from patients with oral inflammation and pneumonia, and then estimate the impact of the bacteria on IgA.The investigation was carried out at the Microbiology Department of the university. 50 samples were taken from individuals with mouth ulcers who had discomfort and redness around the ulcers, as well as symptoms of pneumonia, which were detected on X-rays. The patients were made aware of the research and gave their permission before the swabs and samples collected. Aseptically obtained samples included mouth ulcer swabs and transtracheal wash. Using a disposable 5 ml dropper, we collected the stimulated saliva and then quickly frozen it in a refrigerator (-10oC). We used the ELISA technique to quantify the IgA in saliva, the kit purchased from Mybiosource company, the method was done according to the manufacturer instructions. Also, 5 ml of blood collected from patients with pneumonia for IgA estimation. PCR method done by using primers to determine the genotype of the rmpA gene. It was determined that the patients provided a total of 50 samples for this investigation. Results showed that 39 (78 percent) of the samples tested had bacterial growth, 14 of them were diagnosed as K. pneumonia , whereas 11 (22 percent) of the samples tested negative for growth. The PCR results revealed the amplification of the tested rmpA gene (85.7%) in the oral samples, whereas the percent of pneumonic patients samples was 88.9% and 92.9% of oral plus pneumonic samples. The results showed that no correlation between infection with K. pneumonia with IgA levels in patients with oral lesion , while it showed significant increase in the IgA levels in patients with pneumonia.
In conclusion, The prevalence of k. pneumonia was important pathogen detected in oral, lung or both and it cause increase in IgA levels in patients with pneumonia.