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Goat milk fat is of prime importance because of its beneficial effects on human health. Twelve lactating Damani goats of same age, body weight and lactation number were selected and distributed into 4 groups (n=3). Trial was continued for four weeks with adaptation period of a week. Animals were allowed to graze for 06 hours on natural grasslands each day i.e. Control diet (CD-Diet) while other 3 groups were offered with Rape seed cake (RS- Diet), cotton seed cake (CS-Diet) as well as maize cake (MC- Diet) along with Diet-CD respectively. Medium chain fatty acids (MCFA) are caproic, caprylic as well as capric acid. Capric acid was recorded highest (P<.0001) (10.1±0.006%) for MC- Diet and lowest (9.31±0.006%) for CD-Diet. Mono unsaturated fatty acids (MUFA) including Myristoleic acid, Oleic acid, Elaidic acid etc. Myristoleic acid were recorded highest (P<.0001) (0.11.±0.006%) for CS- Diet and lowest (0.6.±0.006%) for CD-Diet. Poly unsaturated fatty acids (PUFA) are Linoleic acid, Linolelaidic acid, Linolenic acid and Arachidonic acid etc. Linoleic acid was recorded highest (P<.0001) (3.29.±0.004%) for MC- Diet while lowermost was recorded (2.69.±0.004%) for CD-Diet. Saturated fatty acids (SFA) including Stearic acid, Archidic acid, Palmitic acid etc. Stearic acid was recorded highest (P<.0001) (14.9.±0.006%) for CD-Diet its lowest values were recorded (7.76±0.006%) for CS- Diet. It was notice that oil seed cake supplementation have significantly improved the MCFA, MUFA and PUFA while the percentage of SFA was observed lower in the milk of treatment groups animals.