Antimicrobial efficacy of Aqueous Nigella sativa, Aqueous Neem leaf extract, 3% Sodium hypochlorite, and 2% Chlorhexidine against endodontic pathogens belonging to different categories. – An in vitro Study

Abstract

Aim: To evaluate the antimicrobial efficacy of aqueous Nigella sativa against planktonic forms of endodontic pathogens belonging to different categories.

Materials & Methods: Following irrigating solutions were employed

1. Herbal extracts (Aqueous Nigella sativa and Aqueous Neem leaf extract)


2. 3% Sodium hypochlorite (NaOCl)


3. 2% Chlorhexidine.

An agar culture plate was inoculated with three endodontic pathogens belonging to different categories; Gram-positive cocci Enterococcus faecalis (ATCC 29212), Gram − ve Pseudomonas aeruginosa (ATCC 27853) bacterial and fungal cells Candida albicans (ATCC 10231); zone of inhibition of microorganism was analyzed using well diffusion method.

Conclusion:

Within the limitations of this study, Herbal extracts (Aqueous Nigella sativa and Neem leaf extract) showed better antimicrobial activity.