Evaluation Of Glucagon Like Peptide-1 Receptor And Insulin-Like Growth Factor-1 Genes Expression In Type 2 Diabetic Patients Depending On Their Body Max Index

Abstract

Type 2 diabetes mellitus (T2DM) is a complicated metabolic condition caused by a combination of many factors .
Methodology: A total 90 specimens of blood samples have been collected, including (75) samples from patients with T2DM from Al-Shaheed Fairooz Hospital and Al-Kut Hospital in the governorate of Wasit /Iraq, and (15) samples from healthy control individual without any history of T2DM during January to March 2021. The patients’ ages were ranged between 18 and 70 years old.HbA1C test was done for a 75 sample of patients to determine glucose level . Body mass index was estimated for all Patients .The results showed that smoker had higher total HbA1c values than no smoker . As well, It was shown that patients with family history had higher total HbA1C values than patients with not any Family history with T2DM, and BMI showed significant increase in T2DM .
To evaluate the IGF-1and GLP genes expression, performed by RT-PCR technique .The results revealed that there was ( 0.764 ±0.28 ) in GLP-1Rand ( 0.847 ±0.32 ) in IGF-1 expression in all when compared to a healthy control group of patients (1.00 ±0.00 ) . The gene expression according to BMI was showed (overweight =-0.361 , obesity= -0.410 ,P value<0.05) in GLP-1R gene and (overweight =0.72, obesity= 0.75,P value <0.05) in IGF-1 gene .