Comparing Of Staining Giemsa Dilutions For Rapid Detection Of Malaria Parasites At Thick And Thin Blood Smears In Biak And Abepura General Hospitals, Papua, Indonesia

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Yohanna Sorontou , Agussalim

Abstract

Background. The Giemsa stain has been used as gold standard of staining blood smears for diagnosis malaria parasites. Method of Microscopic examination of thick and thin blood smears could be developed become an accurate methods with the effective time so that the patients can be take result examination.


    Objective. To determination and evaluation a rapid methods of the diagnosis of malaria infection by microscopic of stained thin and thick blood smears with modification staining of Giemsa dilutions were compared with standard Giemsa  based on time.


    Methods. This research are quasi experiment study with observation design just looking for three methods are effective with five different times which used in microscopy examination of malaria parasites. The Sample thin and thick blood smears were stained with modification staining of 1:4, 1:5 and 1:6 Giemsa dilution and compared with standard Giemsa staining 1:3, 3% and 10%, with ph 7.2 based on time.


  Result. A total of 130 malaria positive cases, 65 (50) patients were found to carry P. falciparum and 65 (50) carried were P.vivax. The results of this study was indicated that the higher the dilution of the Giemsa solutions, the reduced parasite of malaria.


 Conclusion. Giemsa dilution method that can be used for staining malaria preparations, thick and thin blood smears rapid and accurately in detecting the parasites density of malaria was 1:4 with a duration time of 15 - 20 min, while the 1:5 and 1:6 methods were staining time 5 - 10 min cannot be used because the parasites density of malaria was reduced as result of incomplete lysed of erythrocytes and half parasites were covered by Giemsa.


 

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