Cytotoxicity and Acetylcholinesterase inhibitory and PRAP activities of the essential oils of selected Tanacetum L. species.

Abstract

Essential oils obtained from flowers of Tanacetum mucroniferum Hub. - Mor. & Grierson, leaves of Tanacetum densum (Labill.) Heywood ssp. eginense Heywood, stems of Tanacetum densum (Labill.) Heywood ssp. sivasicum Hub. - Mor. & Grierson were investigated for their cytotoxicity, AChE inhibitory and PRAP activities. We have previously investigated chemical composition of these oils and we have also suspected that these oils also do have strong AChE inhibitory activities due to the insecticidal activities which we have reported for the other members of this genus. Highest activities were observed for pure oils: T. mucronifeum, T.densum ssp. sivasicum and T. densum ssp. eginense were 100.00±0.0%; 100.00±0.0% and 91.31±3.30% respectively (n=3). The observed activities were concentration dependent. Compared with the positive control α-tocopherol (3.735±0.063 AU; n=3) in same concentrations, all of the investigated oils showed low PRAP activity at the highest concentration (10 mg/mL). Highest activity was observed for T. mucroniferum (1.319±0.017 AU; n=3) at 10 mg/mL concentration.