Stability Studies Of Microencapsulated Chitinase, Screened From Marine Bioluminescnce Bacteria- Vibrio Alginolyticus.

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MARSHIA. A , MARTIN. P, AGASTIAN. P

Abstract

Marine bioluminescence bacteria, Vibrio alginolyticus was tested for its enzyme secreting potential. The marine origin enzyme tests were done chiefly for amylase, chitinase, lipase and protease out of which the bacteria showed higher potential activity for the enzyme chitinase. The zone clearance test was done to check for the enzyme activity. The highest value recorded for the chitinase activity was clearance of 2.2 centimeters by the bacteria and proved to be the most potent, among the three other enzymes. These were further studied for its media, pH and temperature optimization, Chitinase activity was better in BOSS broth in pH 5.0 and at temperature 26°C. As chitinase was highly sustaining in these optimal conditions they were furthermore subjected to enzyme immobilization in sodium alginate and calcium chloride beads. The microencapsulated enzyme was compared with the uncoated chitinase enzyme and observed for its activity in different pH and temperature for 12 months. The pH ranges analyzed are 3.5, 7.0 and 9.0 and the temperature in which they were maintained was 4°C, 37°C and 60°C.

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