Evaluation of Agronomy and Resistance Stability of Transgenic Sugarcane with Mosaic Virus-resistant through Pathogen-derived Resistance Approach

Abstract

Sugarcane mosaic virus (SCMV) is one of important sugarcane disease that caused mosaic symptom and yield loss due to mosaic disease reaches 20% at disease incidence rates above 50%. Pathogen Derived Resistance (PDR) is a molecular mechanism that disrupts pathogen expression in host plants. Transgenic sugarcane has been obtained using the PDR (Pathogen derived resistance) technique that expresses SCMV-coat-protein established, and has been tested to be resistant to SCMV until the third generation. This study aims to determine the resistance stability of transgenic sugarcane by considering growth and yield at SCMV post inoculation. The result showed that the resistance character of transgenic sugarcane to SCMV virus was inherited to the next generation (the 4th generation). The resistance of transgenic sugarcane was confirmed by molecular analysis using the RT-PCR method with a capsid protein-based gene targeting to explain the symptoms caused by SCMV. The DNA fragment of capsid protein has been successfully amplified in all sugarcane lines, proving that the symptom that appeared on the leave was caused by SCMV. This research showed that line B10.3 and B11.3 had better growth performance compared to the other transgenic line, Cane yield and sucrose content were also high and concluded that this line was resistant to SCMV infection and had genetic stability. On the other hand, the hight incidence percentage in line B11.1 and B10.2 did not affect the growth and production. The possibility of recovery mechanism from SCMV infection occurred in transgenic sugarcane lines B11.1 and B10.2 during the grand growth phase after infection resulted a normal growth performance. The recovery mechanism in transgenic sugarcane using pathogen-derived resistance approaches need to be proved with experimental research.