Molecular Identification And Characterization Of Nor C Virulence Gene Of Staphy Lococcusaureus Isolated From Children With Tonsillitis

Abstract

Tonsillitis is an infection of the upper respiratory tract that mainly affectpediatric and mostly caused byStaphylococcus aureus that considered one of the most common pathogens that cause tonsillitis, and its importance is due to its antimicrobial resistance and persistence in the internal tissues of the tonsils. In this study, we evaluated more than 120and then selection 120 specific tonsils that were surgically detached from patient with a history of repeated tonsillitis. The tonsil were exposed to microbiological investigation to detect S. aureus. Separates were recognized by PCR genes. Antimicrobial. Isolation sensitivity was determined by disk diffusion tests.

    Totallyseparates were exposed to a polymerase chain reaction and then whole genomic DNA was extracted from 120 Staphylococcus aureus isolates to detectnor Cgenes.Genetically Identification of S.aureus by nor C gene this result revealed that all S.aureus gave positive reaction with molecular size 216bp on agarose gel after electrophoresis, the prevalence of nor C gene among 120 S.aureus isolates achieved by PCR technique by using specific primer. The similarity analysis showed different types of isolates. More than inheritance. Different isolates were recognizedsince the similar patient and likeseparates were acquired from changed patients.