The Effect Of Salicylic Acid And Mannitol On The Propagation Of The Adventitious Shoot Buds Of The Date Palm Owidicv. In Vitro

Main Article Content

Khaun Ali Muhsen

Abstract

This study was conducted in the tissue culture laboratory of the date palm Research Center, University of Basrah, during the period 2/1/2018 to 31/12/2019. It investigates four concentrations of  salicylic acid (SA) and mannitol (MA) (0, 1, 2 and 3) mmoleL-1. It also investigates the effects of all these concentrations on the development of vegetative buds of date palm Owidi cv. In vitro.


The results of this study revealed that buds cultured on a medium supplemented with SA at 2 mmolL-1 had the highest fresh and dry weights, which were 12.60 and 1.23 g, respectively, and had significant difference when compared to other treatments, with the exception of SA at 3 mmolL-1for the fresh weight.


 Furthermore, culture medium supplemented with 2 mmolL-1 SA or 1 mmolL-1 MA showed significantly higher buds number, with 20.28 and 19.5, respectively,when compared to the control.


The results indicated that when media was supplemented with MA at all concentrations, the content of proline and phenolic increased significantly, whereas when media was supplemented with SA at all concentrations, the content of proline decreased significantly.


In the elongation stage, SA and MA at all levels significantly increase the length of adventitious shoot buds and the content of chlorophyll and carotene compared to the control., with the highest length observed in SA at 3 mmolL-1 with 8.8 cm, while with 6.70 and 0.024 µgg-1 FW, respectively for chlorophyll and carotene in MA at 2 mmolL-1.

Article Details

Section
Articles