Immunomodulatory Potential Effects Of Pomegranate On Rbl-2h3 Cells

Abstract

Mast cells have a role in a variety of inflammatory and immunological responses and release a variety of mediators, including histamine.Pomegranate fruit is utilized in traditional medicine of several Asian cultures to cure a number of diseases.Pomegranate biological effects has been extensively studied, including in vitro, in vivo, and in clinical trials.The aim of this study was to evaluate whether pomegranate modulates histamine production using RBL-2H3 cell lines after treated by lysophosphatidic acid (LPA).Histamine levels were measured using a spectrofluorometric approach after whole squeezed pomegranate juice was administered to RBL-2H3 cell lines treated with LPA.We found a significant reduction in histamine level with pomegranate juice treatment in dose dependent manner (p ≤ 0.01).Moreover,there was a dose-dependent slight increase in cytotoxic effects in RBL-2H3 cell lines after pomegranate treatment, but no significant decrease in cell viabilitywas found.Our finding suggests the potential role of pomegranate on the reduction of histamine level induced from mast cell, which indicate to the implication of pomegranate in allergy therapeutic and pharmacology industryfuture work.