Diagnosis of a Local Isolate of Cyanobacteria (Fischerella Musciola) and Study of its Activating Efficacy against some Pathogenic Bacteria

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Rasha Khttab Omar, Yousef Jabbar Ismael Al- Shahiry

Abstract

 The current study included isolating a local strain of cyanobacteria (fischerella) from the local environment (Mosul Dam Lake), and a molecular diagnosis was made for this strain to ensure its genetic classification and diagnosis. The exact scientific name for this strain is (musciola fischerella). Different alcoholic extracts (organic extract, ethanolic extract, acetone extract) were prepared from a biomass of the studied isolates. Specific concentrations of these extracts were tested on pathogenic genera of bacteria isolated from local hospitals of Mosul city (staph aureus, proteus mirabilis, klepsella pneumonia, Escherichia coli and pseudomonas aeruginosa).


 The results showed that the organic extract was the best regarding its ability to inhibit the growth of the studied pathogenic bacteria, as the diameter of the inhibition was (28) mm for bacteria (staph aureus) when using the concentration (250) µg/ml.


 The ethanolic extract had less effect on pathogenic bacteria than the organic extract, and the maximum inhibition was (11) mm against the pathogenic bacteria S.aureus, (9) mm for P.mirabilis, (9) mm for k.pneumonia, (7) mm for P.aeruginosa, and (6) mm for E.coli, respectively, when using the ethanolic extract at a concentration of (250) µg/ml.


 The acetone extract did not give any noticeable positive results, only at the concentration of (250) μg/ml, as the diameter of inhibition was (12, 11) mm for pathogenic bacteria (S.aureus and P.mirabilis) respectively, while (k.pneumonia, E.coli and P.aeruginosa) bacteria did not show any noticeable effect for all concentrations used of the acetone extract.


 

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