Qualitative And Quantitative Phytochemical Screening Of Vitex Negundo L . Extract Using Chromatographic And Spectroscopic Studies

Main Article Content

S. P. Preetha, , R. Arul Salomee Kamalabai , and K. S. Jayachandran

Abstract

In the present study, the plant was identified as Vitex negundo (L) and assigned a voucher specimen accession number as SJCBOT2701. The shade dried leaf powder was extracted with water, and organic solvents such as ethanol, and ethyl acetate. The metabolic extractive value was comparatively higher in the aqueous extract (11.2%) when compared to ethanol (8.6%) and ethyl acetate (6.7%) extracts. Qualitative analysis of extracts revealed the presence of alkaloids, flavonoids, steroids, tannins, coumarins, phenols and terpenoids, and quantified for its phytochemical constituents with High performance Thin Layer Chromatography, Liquid Chromatography- tandem Mass Spectrometry and Gas Chromatography Mass Spectrometry. The results revealed the presence of dodecanoic acid, coumarine-3-carbohydrazine, tetradecanoic acid, trihydroxy isoflavone, hexadecanoic acid, 12-octadecenoic acid, 11-heneicosanone and high amount of flavonoids such as chalcones, flavones, flavonols and isoflavones. The presence of flavonoids and other secondary metabolites may be attributed to the pharmacological activities of V. negundo. Comparison of results with other reports revealed that the secondary metabolites present in the shrub varies from place to place and environment to environment which might be due to the environmental and ecological factors.

Article Details

Section
Articles