Molecular And Biochemical Study Of Serratia Marcescens Isolated From Sheep In Baghdad Province

Abstract

Themolecular and biochemical study for Serratia marcescensisolated from infected sheep in Baghdad province. One hundred and fifty samples from feces of sheep collected from University of Baghdad/College of Veterinary Medicine,Agricultural Research office/ Ruminant Station department, Abu-Ghraib zone, Al-Shu'ala zone, Al-Mahmodia zone, Al-Askan zone in Baghdad province, cultured by pre-enrichment, enrichment, selective and differential media then Gram stain were done;After thatcolonies were cultivated on nutrient agar and incubated at 37°C for 24-48 hours, then examined for form using the naked eye; after purification of cultured bacteria, biochemical tests were done including: Ureas, Oxidase and Catalase, Simmon citrate, and confirmed by Vitek2system and PCR assay16S ribosomal RNA gene (16S rRNA).
Results showed that 7 (4.66%) samples out of 150 samples were positive for Serratia marcescens and it’s the first time to isolate from fecal samples of sheep in veterinary medicine field.