Thidiazuron Induced Plant Regeneration Via Organogenesis And Somatic Embryogenesis In Broccoli Brassica Oleracea Var. Italica

Main Article Content

Raghad M. Abdullah , Shifa M. Salih , Qutaiba S. Al-Nema

Abstract

Broccoli, Brassica oleracea var.italica is an important plant due to its flavor and anticancer activity that received more attention in recent years. The leaf, stem and root explants of aseptic 15 day old seedlings, grown on MS medium were used for callus initiation.Theseexplants were cultured on agarsolidified MS medium containingBAP, NAA, TDZ plant growth regulators.Stem segments gave the highest rate of callusinduction that reached 98% on MS medium supplementedwith 0.2 mg L-1 NAA and 0.5 mg L-1BAP. Also,callus had been initiated from root segments cultured on MS medium fortified with 1.5, 2.0 mg L-1 TDZ. The results indicated that shoot regeneration occurred after sub culturingthe callus in the same medium as for callus induction, in addition to MS medium supported with 4.0 mg L-1BAP and 0.5 mg L-1 NAA. Moreover, different somatic embryos stages (globular, heart, torpedo and cotyledonary) were observed when stem callus wascultured on MS medium supplied with 0.5, 0.4 mg L-1 TDZ. In vitro regenerated shoots derived from callus and those that developed from somatic embryos were transferred to MS medium free from growth regulators as well as MS medium containing 1.0, 2.0 mg L-1IBA for rooting. Rooted plantlets have beensuccessfully acclimatized. An efficient approach was used for inducing shoot regeneration in broccoli Brassica oleracea var.italica via organogenesis and indirect somatic embryogenesis. This research proved that TDZwas effective in inducing somatic embryos from stem and root callus.

Article Details

Section
Articles