Effect Of Gravity Components On The Basipetal Flow Of Endogenous Content Of IBA During Culture Of Coleus Blumei L Stem Cuttings

Abstract

In an earlier contribution the role of gravity components on the initiation of adventitious roots and their subsequent growth on Coleus blumei L stem cuttings has been established (Boissya and Das, 1993). An attempt has been made to find, if any, the role of gravity components on accumulation of endogenous IBA at the base of Coleus blumei L stem cuttings during culture in water for four days kept at various inclinations viz. 22.50,450,67.50 900 and 1800 respectively.
IBA contents at the base of the cuttings were measured at the initial start and after four days of culture at various inclinations by TLC method using ‘Iatroscan TH-10’ scanner.
After four days of culture amount of IBA present at the base of the cuttings showed variation at different inclinations. The highest (50μg/g F.W.) amount of IBA was found to be accumulated at 22.50 inclination, which was followed by 450 inclination (43μg/g F.W.), where early root initiation took place. This had probably suggested that at different inclinations of the cuttings, each of the gravity components pgsinθand pg cosθ had exerted differential effects on the rate of flow of endogenous to the basipetal end of the cultured cuttings depending on their inclination. Of the two components the relatively more effective one decide the net accumulation of IBA at the base of the cuttings. The effectiveness of any of the components was decided by θ, the inclination of cultured cutting. Further examined whether IBA(60ppm), IAA(70ppm) and Kinetin (30ppm) through the bases of the cuttings could influence the relative effectiveness of either pg sinθ orpgcosθ. Results clearly suggested that rate of flow of indigenous IBA from the apex to the base of the cuttings was governed by relative effectiveness of gravity components which was in turn decided by the inclination of Coleus blumei L stem cuttings. Externally supplied growth regulators, however, failed to exhibit any effect on the relative effectiveness of any of the g components viz. pgsinθand pgcos θ.Thus distinctive effect of each g components on the flow of endogenous hormone had been established.