The Prevalence of Brucella Melitensis among health worker, butchers and livestock holders in district Abbottabad, Khyber Pakhtunkhwa, Pakistan

Abstract

Brucellosis is a prolonged contagious bacterial sickness affecting human beings and several sort of native and wild animals. The aim of this study was to find out the prevalence of brucellosis among health care person in district Abbottabad. The current study was conducted at Veterinary Research and Disease Investigation Centre Abbottabad. During this study 200 samples were run in different times of fractions. The data about risk factors linked with brucellosis was collected on a pre-designed questionnaire. Initially all the collected samples were diagnosed for antibodies against brucella by serum plate agglutination test and rose bangle plate test. The Seropositive samples were confirmed by Enzyme linked immunosorbent Assay (ELISA). RBPT and SPAT being screening test proved maximum number of positive cases which were when tried through ELISA showed some negative results. Out of 200 cases the positive case were 52 through RBPT and 50 through SPAT. The ELISA eventually when conducted out of 200 samples only 18 were positive. This shows high specificity of ELISA. In terms of overall seroprevalence percentage the present study depicted that brucellosis was 26% through RBPT and 25% through SPAT and 9% through ELISA. Most of the cases which were positive grouped in the age between 21 to 40 years. Professional and occupational workers are more prone to the infection in case of the present study; mostly positive cases were occupational workers and professional individuals.