Production of Polyclonal Antibodies against Exotoxin A of Pseudomonas aeruginosa

Abstract

            Pseudomonas aeruginosa is Gram- negative opportunistic pathogen that causes chronic and acute infections at different sites within body mostly in hospitalized patients such as urinary tract infections, respiratory system infections, dermatitis, soft tissue infections and bacteremia. Exotoxin A inhibits protein synthesis in infected cells by ADP-ribosylation of eukaryotic elongation factor 2.  Exotoxin A one of the most virulent factors of Pseudomonas aeruginosa which was concentrated and purified. Purification of toxin A produced by only two steps ion exchange chromatography and gel filtration chromatography which yield highly purified toxin. The molecular weight of exotoxin A is 66 KDa. ELISA is used for diagnosis of exotoxin A which act as more rapid, sensitive and simple methods .SDS, indirect ELISA and Western blotting test performed to confirm extraction of toxin A. Special primers designed to detect presence of tox A virulence factor, 70.5% of isolated samples have the toxA gene.