Antifungal Activity Of Purified Dextrnase From Dental Caries Staphylococcus Salivariusagainstoral Candidiasis Caused Bycandida Albicans

Abstract

Dental caries from the most populardental illnesses, and it is caused by chronic infections of the normal flora in the mouth. Cavities are created by
acidic metabolites produced by surviving bacteria found in the mouth that feed on sugar.When the dextran used as a substrate
sevenStaphylococcus aureus and Staphylococcus salivarius isolatesisolated from dental caries can secret extracellular dextranase with potential
index for ranger between 0.78-1.72. An ammonium sulfate, DEAE-cellulose column and then sephadexG-150 column were used to purify of
dextranase with a final recovery 32.3%. Ten Candida albicans isolates were isolated from patients infected with oral candidiasis and these fungal
isolates revealed high level of antifungal activity with MIC values ranged from 25 to 50 μg/ ml. In contrast, the fungicidal effect of dextranase
appeared against Candida albicans isolates with concentrations values from 50-100 μg/ ml, thus the dextranase may be an alternative promising
treatment for fungal infections caused by Candida albicans such as oral candidiasis.