Consistency Of Endo-Rhizobacteria Formulation As Biocontrol Ingredients For Fusarium Disease In Shallots (Allium Ascalonicum L.)

Abstract

Fusarium oxysporum is a serious disease that affects shallots and can result in crop failure. Controlling fusarium
disease requires a variety of strategies, including the development of microbial antagonists. Microbes are used
sparingly in the field due to their instability, inconsistency, efficacy, and shelf life. The purpose of this study is to
determine the efficacy of endophytic and rhizobacteria formulations in carrier media as biological control agents
against fusarium disease in shallots.Protease, amylase, and inhibition tests were performed in vitro on three
bacterial isolates formulated on 0.85 percent NaCl carrier, talc, bentonite, and husk charcoal powder. The
variance of the data observations was determined, and those that revealed a significant effect were further
examined using Duncan's Multiple Range Test at a 95% confidence level.The results indicate that rhizobacteria
and endophytic bacteria formulated in the carrier medium are capable of inhibiting Fusarium oxysporum
pathogen development and increasing the activity of protease and amylase enzymes. Protease and amylase
enzymes exhibited the greatest inhibitory power and activity when used to treat the W2R06 isolate rhizobacteria
formulation that was formulated in a carrier medium of husk charcoal powder. W2R06 isolate formulated in
husk charcoal may be used as a biological controller for plant diseases, particularly fusarium disease, thereby
reducing the need for synthetic pesticides.