Molecular diagnosis of co-infection between Giardia lamblia parasite and Helicobacter pylori bacteria by Nested-PCR in patients from Wasit Province, Iraq

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Prof. Dr. Abdulsada A. Rahi , Raheeq F. Kadhim

Abstract

The present study was performed to identify the mixed infection of Giardia lamblia parasite in correlation with Helicobacter pylori bacteria in patients from Wasit Province, Iraq. The study included 72 stool samples ; 24 healthy individuals as control , 24 patients with G. lamblia (GL group), and 24 patients with H. pylori (HP group), collected during January to July, 2021 at Al-Kut General Teaching Hospital, Kut-Iraq. All previously samples were tested using stool antigen test and microscopy. In the present work, the GL and HP groups were examined using a nested polymerase chain reaction (NPCR) method for the detection of G. lamblia and H. pylori in the patient stool samples. According to the NPCR test, the results showed that 14 (58.3%) patients were infected with G. lamblia, and 21 (87.5%) patients were infected with H. pylori. For the detection of G. lamblia-H. pylori co-infection, the findings revealed that 16 (66.7%) of the GL patients also were infected with H. pylori, and 22 (91.7%) of the HP patients also were infected with G. lamblia. There was significant (p≤0.05) positive correlation for the co-infection of G. lamblia and H. pylori. The study suggests that H. pylori infection may highly indicated the mixed infection of G. lamblia in clinical cases presented to the Department of Gastrointestinal Diseases, Al-Kut General Teaching Hospital (Kut City, Wasit Province, Iraq).

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