Investigation Of Some Genetic Virulence Factors In Isolated From Different Clinical Enterococcus FaecalisSourcein Kirkuk Governorate Using Polymerase Chain Reaction(Pcr) Technique

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Ali Hussein Abdullah Al-jobury , Prof. Dr. Ibraheamsaleh Al -jobury

Abstract

The presence of Enterococcus faecalisbacteria was investigated in (260) samples taken from different pathological sources, as (120) samples were collected from persons with urinary tract infection, (80) samples from Stool and (60) samples from infected persons. With infection of the root canal of the tooth, and from different age groups, the isolates were diagnosed by biochemical and culture tests, as well as using the APi-20 strep system and using the Vitek2 device,Then the isolates were diagnosed based on the genotype by molecular diagnosis using the polymerase chain reaction (PCR) technique, and 40 isolates belonging to Enterococci bacteria were obtained, based on the initial culture tests, and 22 isolates by the biochemical tests conducted on them. And 12 isolates belonging to the type Enterococcus faecaliswere obtained by diagnosing them with APi for-20 and Vitek2 system, and after conducting a molecular examination (16SrRNA) on them, it was confirmed that 12 isolates of bacteriaE.faecalis The results of detecting the ability of the isolates to produce virulence factors showed that all (12) isolates were not producing Van A factor, but (5) isolates (41%) showed their ability to produce Van B factor. The percentage was distributed as follows: (3) isolates (25%) of tooth root canal infections, (1) isolates (8%) of urinary tract infections, and (1) isolates (8%) of feces.

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