Role Of Commiphoramolmol In Prevention Of Indomethacin Induced Gastric Ulcers In Adult Male Albino Rats (A Histological And Biochemical Study)

Abstract

Background: Herbs used in traditional medicine comprise manycomponents of medical importance. The Anti-ulcer actions of CommiphoraMolmol(CM) were investigated and it was considered to be of value for its antioxidant properties.
Objective: The current work aimed to evaluate the protective effects of CM on indomethacin-induced gastric mucosal ulceration.
Material and Methods: Fourty adult male albino rats were included and categorized into four equal subgroups: Group I (control) received distilled water, group II (Indomethacin-induced gastric ulcers), group III received 250 mg/kg of oral CM for two weeks followed by intraperitoneal indomethacin; group IV received 500 mg/kg of oral CM for two weeks followed by intraperitoneal indomethacin. Indomethacin dose was 30 mg/Kg. At the end, animals were sacrificed and blood samples were collectedto measure tumor necrosis factor-alpha (TNF-α). The stomach was obtained, opened through the greater curvature, washed with saline, fixed by pins over a cardboard plate and inspected for ulcers. Then, it was used for histopathological andimmunohistochemicalassessment.
Results:Administration of CM 250 or 500 mg/kg revealed a marked decrease in the ulcer index, when compared to group II (10.2, 7.6 versus 15.5).Additionally, there was a significant increase of malondialdehyde (MDA), TNF-α and matrix metalloproteinase-9 (MMP-9) in group II than the control group. The use of CM was associated with a significant reduction of all biomarkers than the indomethacin group. The ameliorative effect was more intense witha high dose of CM. However, values did not reach normal levels. Also, pretreatment with CM 250 or 500 mg/kg before indomethacin led to partial preservation of the mucus layer lining of the stomach. A better protective effect was observed with the high dose.
Conclusion: Oral administration of CM led to a protective effect in a dose-dependent manner against indomethacin induced gastric ulcers.