Detection And Extraction of Antibacterial Compounds from The Leaves of Sonchus Asper Plant

Abstract

Antibiotic resistance among different pathogenic bacteria poses a significant threat to human health. The search for novel class of antimicrobials from different resources such as medicinal plants is a continuous effort of the modern-day scientists. The present work was planned to extract and evaluate the antibacterial activity of Sonchus asper crude extracts against multi drug resistant bacterial pathogens. Methanolic, ethanolic and aqueous extracts dissolved in DMSO have been tested against multi drug resistant human’s bacterial pathogens, including Gram-positive bacteria Staphylococcus aureus ATCC 43300 and Bacillus subtilis subsp. B. spizizinii ATCC 6633, Gram-negative bacteria Escherichia coli ATCC 25922 and Pseudomonas aureginosa ATCC 27853. The well diffusion method was used by measuring the zone of inhibition of bacterial growth from the plant extracts while DMSO as negative control and streptomycinas a positive control. All three extracts showed good antibacterial activity. However, methanolic extract of S. asper showed maximum antibacterial activity against S. aureus, while zones of inhibition for B. subtilis subsp. B. spizizinii, E. coli, and P. aureginosa were also significant. Furthermore, FTIR spectroscopic analysis for methanolic extract of S. asper showed the presence of various organic bioactive compounds. It is concluded that S. asper has crucial medicinal significance, and advanced molecular-level analysis of its components may provide a basis for the production of clinically important natural antimicrobials against various pathogenic and resistant bacterial strains.