In Vitro Production of Cestrum Nocturnum L. Plants for Drought Tolerance

Main Article Content

Hoda I. M. El-Gedawey, Ahmed Abdelkhalek, Mohamed K. Gaber

Abstract

This experiment was carried out on Cestrum nocturnum plants at the tissue culture laboratory, Faculty of Agric. Saba Basha, Alex. Univ., during the 2019 and 2020 years to find a reliable protocol to investigate the micropropagation of different PGRs (KIN, BA and NAA) and to progress the plant's in vitro micropropagation ability under different levels of salinity. In vitro,MS culture media supplemented with BA and KIN at 0.25 mg/L, and NAA at 0.50 mg/L yielded the best results in vitro for both the shooting and rooting stages. For the enhancement of in vitro propagation ability under salinity stress, shootlets of C. nocturnum L. plants grown on MS media supplemented with 0.5 g/L NaCl + 5 or 10 g/L mannitol survived at 100%, but 91.0 and 89.36 percent, respectively, resulted from 1 g NaCl + 5 or 10 g/L mannitol, which nearly survival values were equaled with the control treatment.

Article Details

Section
Articles