Polymerase Chain Reaction Of Oprl And Oprigenes In P. Aeruginosa Isolated From Burn And Wound Infections

Abstract

Background and objectives: P. aeruginosa owned diverse virulence factors, completely factors contributed to increase its pathogenicity as outer membrane proteins. The goal of the current study was to detect oprI and oprL genes like creditable factors for quick identification of the P.aeruginosa by multiplex PCR technique.
Materials and methods: P.aeruginosa isolates were isolated from 50 burn and wound patientsfrom Teaching Hospital of Mussan province/Iraq during the period from March to May, 2017. The samples were identified by several selective media to detected the morphological characteristic and by biochemical tests.
Result: The using of PCR technique showed all isolates (100%) had oprI gene while 83.33% isolates harbored oprL gene.