DISTRIBUTION OF ALLELES AND GENOTYPES OF CTLA4 AND TNFΑ POLYMORPHISM IN PATIENTS WITH AUTOIMMUNE THYROIDITIS

Main Article Content

Rana Rahimova, Gulnara Dashdamirova, Sabina Guliyeva

Abstract

aimed to examine the frequency of alleles and genotypes of CTLA4 and TNFα gene polymorphism in patients with autoimmune thyroiditis.


Under supervision were 170 patients with autoimmune thyroiditis (AIT), 64 men and 106 women, aged 18 to 64 years. The control group was 65 people without thyroid pathologies and other autoimmune diseases aged 20 to 65 years, of which 26 were men and 39 were women.


Genotyping of single nucleotide polymorphisms CTLA-4 and TNF-α was carried out based on the genetic laboratory of the YASHAM clinic.


For molecular genetic analysis on polymorphisms of loci CTLA4 (rs231775) and TNF, real-time PCR was performed with fluorescently labeled probes on the CFX96 (BioRad) instrument, followed by visualization and interpretation of the results in the Bio-Rad CFX-96 program.


Statistical analyses were performed using Statistica 12, Microsoft Office Excel, and Microsoft Office Word 2010.


Analysis of the distribution of allele frequencies and polymorphism genotypes rs 231775 of the CTLA-4 gene among AIT patients revealed a statistically significant increase in the occurrence of the "rare" G allele (48%) compared to control group (33.8%), p < 0.05 ( χ 2 = 4.27, OR = 1.865, 95% CI = 1.028-3.382) and a decrease in allele A, (51.2%) relative to control group (66.1%), p < 0.05 (χ 2 = 4.27, OR = 0,536, CI = 0,296,-,0,973).


Carriers of the G allele and the GG genotype have an increased risk of developing AIT, while carriers of the A allele have a reduced developing risk of this disease. The G allele and AG, GG genotypes of the TNF-α gene 308AG polymorphism are risk factors for the development of AIT and therefore genetic markers of AIT. Analysis of the frequency of occurrence of allele A and genotype AA showed a statistically significant increase in both study parameters in individuals in the control group, 73.8% and 64.6% compared to patients with AIT, in whom these values were 57.1%, respectively, for allele A (p = 0.0179, χ 2 = 5.61, OR = 0.471, 95% CI = 0.250 - 0.885) and 24.1%, for the AA genotype (p = 0.0000, χ 2 = 33.76, OR = 0.174, 95% CI = 0.094-0.323).

Article Details

Section
Articles