Evaluation Of Serum Levels Of Cyclooxygenase-1 (COX-1) Among Patients With Type Two Diabetes Mellitus (T2DM)

Abstract

Type 2 diabetes results from a combination of genetic variants associated with insulin resistance and decreased insulin secretion, as well as environmental factors such as obesity, overeating, lack of exercise, stress, and age. the study aims to explore the association between serum levels of COX1 and diabetes mellitus. The study included 40 patients with type2 DM(20 males and 20 females ) and their age range between and 40-80 healthy individuals. The amounts of cyclooxygenase-1in serum were determined using the enzyme-linked immunosorbent assay (ELISA) method. As control group and their age range between40-80(10 males and 10 females). Blood samples were collected from patients and control groups. The sample was divided into two parts, the first part was subjected to Trizol preservation and the other part for serum separation. The results of this study showed a decrease in the concentration of cyclooxygenase-1 (COX-1) in the sera from patients with T2DM compared with the healthy participants. The level of the patients was (0.1029 ± 0.0233) compared to the control group (0.121 ± 0.0361), P = 0.047. There was no significant difference when comparing the concentration of COX-1 among males of the studied groups: patient group 0.099 ± 0.0215 versus control group 0.1023 ± 0.0157, P = 0.642. Females with T2DM revealed a decrease in the concentration of COX-1 with a significant difference compared to the females of the control group 0.1068 ± 0.0250 versus 0.1405 ± 0.0411, P = 0.034 of the two groups respectively. No significant difference in the concentration of COX-1 corresponds to males and females of the patient group. According to the results of the current study, we conclude that: COX-1 is a major contributor to the onset of type 2 diabetes and there may be an association between low levels of cyclooxygenase-1and type 2 diabetes.