Serum TNF-Α Concentrations In Type Two Diabetes Mellitus

Abstract

Diabetes is one of the most common metabolic diseases, and its incidence continues to increase in many parts of the world. It is estimated that more than 90% of diabetes is type 2 diabetes mellitus (T2DM), which occurs for two main reasons: a lack of insulin production by β-cells of the pancreas, as well as peripheral insulin resistance (IR) in many organs such as the liver, skeletal muscles, and adipose tissues. It can be considered that pro-inflammatory cytokines are one of the most important variables that lead to T2DM and also contribute to the development of insulin resistance. Tumor necrosis factor-alpha (TNF-α) is the most important pro-inflammatory mediators, as it causes inflammation in various parts of the body.
TNF-α concentrations in sera were measured by enzyme-linked immunosorbent assay technique (ELISA) using Human tumor necrosis factor-alpha (TNF-α) ELISA Kit (Elabscience Company /china).
Serum TNF-α concentrations displayed a significant difference in T2DM patients when compared with the control group (Diabetic patients 30.36 ± 14.51 compared with control 23.30 ± 7.030, P-value = 0.0149, T-test = 2.489). TNF-α concentrations of males with diabetic patients displayed significant difference when compared with controls (31.83 ± 13.27 versus 22.41± 6.32, P-value = 0.0438, T-test = 2.751). However, the TNF-α concentrations of females of diabetic patients displayed non-significant difference when compared with controls (28.87 ± 77 versus 24.19 ± 7.79, P-value = 0.4377, T-test = 1.069).