Molecular Detection Of Human Parvovirus B19 In ΒThalassemia Patients At Thi-Qar Province/ Iraq

Abstract

Introduction
The thalassemia is known as genetic defects that autosomal recessive, characterized by a reduction or absence of
production one or two types of globin chains. This condition will have an impact on the quantity and quality of
blood produced.Human parvovirus B19 has a significant affinity for hemopoitic stem cells and integrates into a
particular location in the human genome. The infected cell is unable to divide, resulting in a reduction in the
generation of new RBC. Parvovirus is a group of tiny (25nm) non-enveloped viruses. Parvovirus is derived from
the Latin word parvus, which means small. A linear single-stranded DNA (ssDNA) genome of 5 to 6 kb is bordered
by two terminal hairpin formations.
Method
Genomic DNA from blood samples were extracted by using (G-spinTM Total DNA Extraction Kit). The extracted
blood genomic DNA was checked by using Nanodrop spectrophotometer (THERMO. USA), which measured DNA
concentration (ng/µL) and check the DNA purity by reading the absorbance at (260 /280 nm). The Real-Time PCR
technique was performed for direct detection of Parvovirus B19 virus based NS1 region in β-Thalassemia
patients’blood samples.
Result
During the current investigation, blood samples from 100 thalassemia patients were utilized to determine the
presence of Parvovirus B19 ssDNA in blood samples using the qPCR technique. Parvovirus was found in 54 % of
thalassemia patients. Addition, Females were infected with B19v in greater numbers than males (29 vs. 25). The
numbers of patients B19v infected with B19v in the countryside is 44, which is higher than the numbers of
individuals B19v infected in the city, which is 10.In addition, the numbers of B19v infected people in the first age
group is the highest, with 29 patients, followed by the second age group with 20 patients, and groups over 21
with the least number of B19v infected people.The most infected blood type is A +, which has 23 patients,
followed by B +, which has 17 patients, and A +, which has nine people. It is the least common blood type among
the others. In any event, carriers of type AB + are not infected with B19v.

Conclusion
Nat. Volatiles & Essent. Oils, 2021; 8(4): 1895-1909
1896
Human parvovirus B19 infections vary depending on an individual's immunity. May be an O+ is the most
commonly blood type, thalassemia patients who are often frequent blood transferred to become vulnerable to
the risk of contracting the B19 virus.