Specific Detection Of Brucella Spp.In Cattle Slaughtered In The Province Of Bolivar (Ecuador)

Abstract

In this research, we set out to study the prevalence and molecular characterization of Brucella isolates obtained from the blood of cattle slaughtered in 2 herds of the province Bolivar, Ecuador.Therefore, 100 samples of bovine blood were selected, 50 from the Guaranda slaughterhouse and 50 from the Echeandía slaughterhouse.All blood samples were analyzed using the Rose Bengal (RB) serological test, as well as the culture on selective brucella Agar + blood "5% sheep blood supplement" (BAS), under microaerobic conditionsat 37 °C for 24-48 h.Similarly, the isolates obtained by culture were characterized by PCR using a pair of primers that amplify a 905-bp fragment. After the RB serological test, 54% of the analyzed samples were positive, while 63% were positive by culture.Regarding the molecular characterization, 42% of the isolates presented the characteristic band, resulting in being positive for the Brucella genus, being the San Pablo sector the one with the highest prevalence 75%, followed by the Salinas sectorwith 50%, San Lorenzo 47,05% and Echeandía with 31,9%.In relation to the prevalence of the pathogen by sex, this was higher in female cattle.In conclusion, the PCR technique can be considered as a useful molecular tool in the diagnosis of Brucella spp.in cattle, especially due to its greater sensitivity.