Interleukin-1α And Alkaline Phosphatase Gene Expression Towards Osteoblast Cell Culture Post Alpha-Mangostin Exposure (An In-Vitro Laboratory Experimental Study)

Abstract

Currently in the Dentistry field, there are lots of things that are related to the process of bone regeneration. Bone healing deeply relies on the early inflammation phase, which is affected by local and systemic responses towards adverse stimuli. Indonesia has lots of traditional plants that can be used as an alternative for complementary therapy and alternative medicine. The natural substance alpha-mangostin was utilized in this study because earlier research suggested that it has anti-inflammatory effects via cytokine expression and increases osteoblastic differentiation by assessment of multiple osteogenic markers following LPS administration. This study uses a post-test-only control group design to look at the expression of IL-1 and ALP genes in osteoblast cell culture using Real-time PCR. This study was divided into four experimental groups, one control group and three treatment groups. IL-1α gene expression increased with LPS induction (E1), and decreased in a group with α-mangostin (E2) and group with LPS and α-mangostin (E3). Whereas ALP expression decreased in a group with LPS (E1) and group α-mangostin (E2) and remained constant with the control group in the group given LPS and α-mangostin (E3). From this study, it can be concluded that LPS can induce inflammation in osteoblast cell cultures and α-mangostin can reduce inflammation which is characterized by decreased expression of the IL-1 gene, but has not shown any effect on osteogenesis.