Promoter Methylation Status Of Large Tumor Suppressor Gene Family (LATS1&2) And Its Significance As Therapeutic Target In Colorectal Cancer (CRC)

Abstract

Background and Aim: Promoter methylation is an epigenetic modification that downregulates the expression of genes. Both LATS1&2 plays significant role as tumor suppressors. In this study promoter methylation of these genes and correlation with various clinico-pathological characters were studied.
Materials and methods: A total of 65 tumor and adjacent normal tissues were taken for the study. LATS 1 & LATS 2 methylation profiles were investigated using methylation specific PCR (MS-PCR).
Results: The promoter site of LATS 1 & LATS 2 genes were found to be hyper-methylated in 52.3% and 61.5% respectively of the CRC patients. Statistically a significant correlation was observed between LATS 1 & LATS 2 promoter hyper-methylation with Lymphnode metastasis (OR=2.24; 95% CI: 1.40-3.54, p=0.001);(OR=2.87;95% CI: 1.0-8.2, p=0.04).
Conclusion: LATS1/2 hypermethylation is a key step in the development of colorectal cancer and that it could be exploited as a diagnostic biomarker moreover these findings may provide useful insights for the development of CRC diagnoses and treatment.