The Qualitative Evaluation Of Some Amino Acids In Urine Of Children With Nephrotic Syndrome

Abstract

syndrome is one of the common pathological cases in children in Karbala, Iraq. The disorder of amino acids metabolism can be considered as a major cause of the nephrotic syndrome, especially in children, and disorders of protein and amino acids metabolism can include conditions that occur when the body is unable to break down certain amino acids or their production, which leads to the accumulation of toxic substances for some substances or a deficiency in the production of beneficial compounds. This work was included children with nephrotic syndrome (n=50) and healthy children (n=50) within the age group (2-10 years). Samples (urine and blood) were collected for the participants of the study (patients and healthy volunteers).Thin-layer chromatography (TLC) technique was used to identify the amino acids in urine samples, which were conducted in the study (50 healthy, 50 patients) by comparing the distance and the colour of the complex produced by standard solutions with the distance and colour of 20 predicted amino acids. The eight amino acids were identified in urine samples in this study (glutamine, threonine, asparagine, leucine, serine, alanine, glycine). The comparative biostatistical analysis between groups of patients and healthy people in the study showed the following results: (i) the level of glutamine in urine for patients with nephrotic syndrome more than in the healthy children, where the percentage in the infected was 22%, while in the healthy ones was 5%, and the probability value was p = 0.0001. (ii) There are significant differences in the level of asparagine between patients and healthy, where the value of the amino acid for patients was 38%, while in the healthy controls it was recorded 46% with a significant difference at the level of probability (P<0.001), (iii) A significant decrease of serine in the urine of patients, and it was 3% compared with the healthy 22%, with p = 0.0001, (v) Decrease in the percentage of lysine in the urine of patients with nephrotic syndrome16% compared to the healthy 46% with a level of probability (P<0.0001), these results clearly indicate to the relationship of the levels of various amino acids between the children with nephrotic syndrome and healthy children.