Control Of Methicillin Resistant Staphylococcus Aureus By Bacteriophage, Endolysin, Bacteriocin

Abstract

Foot ulcers are the main cause of hospitalization and mortality of diabetic patients over the world. Approxi-mately 25 % of diabetic patients will suffer from wound infections during their lifetime. Resistance of bacterial pathogens to available antibiotics has become a great load to the human health care, resulting in a significant progression of morbidity and mortality. Therefore, the use of novel solutions with antimicrobial properties such as bacteriophages attracted the attention of many investigators. In the current study, twelve multidrug re-sistant (MDR) S.aures isolates were isolated from hospitalized diabetic foot patients and bacterial identification was done based on biochemical and molecular characteristics using 16srRNA. Bacteriophages are a potential tool to treat foot ulcer resulting from biofilm-associated infections. Phage cocktails were used to detect S. au-reusinsusceptibility. An adaptation procedure was performed in cases of bacterial resistance. Bacteriophage with lytic activity against MDR S.aureus. bacteriophage are characterized physically through the evaluation of their lytic activities at a wide range of temperatures (40–90° C) and pH values (2–12) control the rate of adsorp-tion. Transmission electron microscope (TEM) study revealed that phage belong to Podoviridae family. The present study opens a new window for the application of bacteriophages as promising antimicrobial agents against MDR bacteria.