Determination Of Bacteriophages Ability That Isolated From Poultry Feces To Lysis Salmonella Typhimurium That Isolated From Stool Of Children Who Suffering From Diarrhea

Abstract

This study was conducted to isolate Salmonella from 350 stool samples taken from children under five years in age, suffering from diarrhea during the period from March 2019 to March 2020 in Tikrit city / Iraq. The results showed that isolate ten isolates of S. enterica subsp. enterica at infection rate of 2.875% of the total patients suffering from diarrhea, as well as the results showed that the age group of 49-60 months was more susceptible to salmonellosis. The results of the serotyping diagnosis also showed that six isolates belong to the serotype S. Typhimurium and the other belong to the serotype S. Enteritidis. The results of the isolates sensitivity test to antibiotics showed that all isolates were sensitive to the antibiotics Azithromycin, Cefotaxime, Ceftriaxone and Chloramphenicol, while the sensitivity of the isolates to other antibiotics was varied, as 40% of the isolates were resistant to the antibiotics Ampicillin, Ceftazidime and Nalidixic acid, while the isolates were resistant to the antibiotics Gentamicin and tetracycline by 30%, 40%, and 80%, respectively. The results of the study showed the possibility of isolating two virulent bacteriophages that have the ability to infect and kill S. Typhimurium isolates. The results also showed that SPh2 is more tolerant of heat and extreme conditions of pH than SPh1.