Isolation And Identification Of Microbiota Species Isolated From Stool Specimens Among Autism Spectrum Disorder Children In Thi-Qar Province, Iraq

Abstract

Background: Autism spectrum disorder (ASD) is a neurodevelopmental disorder within a numerous neuropsychiatric disorders have been linked to the microbiota.ASD is including anxiety, schizophrenia, autism, bipolar, and depression disorder genetic ingredient that manifests itself in various distinct phenotypes. The term "microbiota" points to the microbial population found within the human body, including bacteria, viruses, archaea, protozoans, and fungi. The combination of bacteria, , and archaea Settle down the gastrointestinal tract is called the 'gut microbiota' and has evolved with the steward over thousands of years to form a complex and exchange advantage relationship.
Method: One hundred autistic patients were enrolled in this study. Fifty healthy individuals were participated in this study. Stool specimens of ASD children were cultured on thioglycolate broth then transfer into selective and differential media (blood agar, Mac Conky agar, EM Bagar, Mannitol salt agar and Chocolate agar) Phenotypic characteristics were identified using API 20 system.
Result: In current study, API20 system was used for identification the isolates. The results showed Enterobacteriaceae members more prevalence such as Escherichia coli (13%), Enterobacter spp. (15%), Citrobacterspp (6%), Pantooeaspp, Serratiaodorifera and Kluyvera spp. (2%). Other bacterial that were isolated Enterococcus spp. 3% while Staphlococcusspp (6%),as well as Listeria spp, Micrococcus ,and Neisseria sicca less common.
Conclusion: It is evident that ASD affects the gut microbiota, although additional research is needed to determine if this is a cause or effect of the disorder.