Effects Of Iba And Ba On Shoots Nods Internodes And Callus Of Ficus Carica Explants In Vitro

Abstract

The study was carried out at the Ministry of Science and Technology that investigated the effects of IBA and BA on Ficus carica explants in vitro, our Data showed significant effect of plant growth concentrations on No. of shoot but the most effective concentration was (0.8mg/l.-1 of IBA) which gave highest no shoot reached (1.32) also adding BA at (8 mg/l.-1or 4 mg/l.-1) produced the highest shoots 1.867, 1.72. In general, 0.8 mg/l-1 of IBA gave highest number of shoots 1.92 for both nodes and shoot tips and there is no significant differences between them. when adding BA at concentration of 8 mg/l-1 was superior to give highest number of shoots 2.96 initiated from nodes explants. The highest callus induction% reached 82.67 % and 74.67% for the combinations of (1.5 mg/l-1 IBA + 4 mg/l-1 BA) and (1.5 mg/l-1 IBA + 8 mg/l-1 BA) respectively. IBA maximum callus induction 49.60 % at 1.5 mg/l-1 and 51.73% for 8 mg/l-1 of BA. In related to the different explants responses internodes recorded 40.48 % as compare with 31.36% and 24.80% for nodes and shoot tips respectively. For the two interactions between IBA or BA with the explants Data showed that 1.5 IBA mg/l-1 gave influenced positively on callus induction from internodes explants 75.20% and 8 BA mg/l-1 affected with percentage of 59.20% and 55.20% on nodes and shoot tips respectively.interactions among IBA , BA with the explants Data showed that highest callus induction percentage 100% for the internodes explants in media MS containing combination of 1.5 mg/l-1 IBA + 4 mg/l-1 BA or 1.5 mg/l-1 IBA + 8 mg/l-1 BA and 88.0% for nodes explants in media MS containing combination IBA + 4 mg/l-1 BA.