Studying Polymorphism Of Toll-Like Receptor-6 And HADV7 In Childhoodwith Acute Respiratory Tract Infections

Abstract

Background:Infection wih HAdV 7 develops serious illness with many complicationsduring childhood. As part of
innate immune response, recognition of pathogen associated molecular patterns is mediated by pattern recognition
receptors including Toll-like receptors (TLRs).
Method:One hundred cases included in the study; 75 with acute respiratory tract infection (ARTI),diagnosed
medically by specialist clinician, their mean age was (40.56±8.31 months) and 25 apparent healthy controls (AHC)
with mean age (43.6± 10.96 months). DNA extracted from blood specimens, nasopharyngeal and throat swabs from
patients and controls for further extraction of human adenovirus (HADV7) genome and total genomic DNA. Detection
of Toll-like receptor 6 (TLR-6) polymorphism detected by using ARMS PCR technique.
Results:HADV7 nucleic acid detected in 66.7% of ARTI cases, while no viral nucleic acid was detected in AHC group
(P=0.03). In ARTI, the most commonly affected age stratum infected with DNA –HADV7 was (6-44 months) which
constituted 34% (P< 0.05).
The results of TLR-6 (rs5743557) amplification appeared that frequency of GG and AG genotypes in patients with ARTI
and AHC groups was significantly increased in patients than controls, in which GG genotype increased as rate
OR=2.489 compared with GA and AA genotypes in studied groups.
Conclusions:HADV7 that recently identified in Iraq causes acute respiratory tract infections in children and more
specific in infants. In addition, TLR 6polymorphism in patients could point for its possible roles in pathogenesis of
acute respiratory tract infection.
Nat. Volatiles & Essent. Oils, 2021; 8(4): 1998-2013