In Vitro Rumen Methanogenesis Inhibition Ability Of Brown Seaweed From Nusakambangan Coast, Cilacap, Indonesia

Abstract

The objective of this study was to examine the effects of Sargassum sp. brown seaweed supplementation on methane gas production, methanogen, and total gas as the main indicator of anti methanogenesis agent. The materials used in this study: Sargassum sp. brown seaweed meal (from Nusakambangan Coast, Cilacap, Indonesia), sheep rumen fluid, and laboratory equipment. Completely Randomized Design (CRD) was used in the experimental research of 6 treatments, each with four replications. The applied treatments were P0 = rumen fluid with basal feed (CP = 11.8% and TDN = 60%); P1 = P0 + 0.03% monensin; P2 = P0 + 1% Sargassum sp.; P3 = P0 + 2% Sargassum sp.; P4 = R0 + 3% Sargassum sp.; and P5 = R0 + 4% Sargassum sp. The result of the experiment showed that the supplementation of Sargassum sp. decreased total gas, methane gas concentration and methanogen, increased dry matter digestibility and organic matter digestibility, and did not significantly affect (P>0.05) protozoa. Supplementation of 2% Sargassum sp. decreased methane gas by 82.95% and decreased methanogen by 75.17%. In conclusion, Sargassum sp. brown seaweed meal was an effective anti methanogenesis agent.